IVF – in vitro fertilization
Fertilization in vitro – out of the woman’s body. The sperm is added to oocytes. The sperm is prepared in laboratory but the fertilization itself is natural (i.e. the sperm and the ovum merge naturally)
Indication:
- normozoospermia resp. light SPG disorder in a man
- sufficient number of oocytes
- previous cycles with proper fertilization of oocytes
ICSI – intracytoplasmic injection of sperm into oocyte
This method is based on the selection of a sperm that is transferred directly into a ripe oocyte
Indication:
- SPG disorder – decreased number of sperm, insufficient movement of sperm, high pathology
- sperm gained from MESA/TESE
- immunological factor – antibody of oocyte against sperm
- idiopathic sterility
- insufficient or no fertilization of oocytes in previous IVF cycles
- repeated IVF cycles
- development of inferior embryos in previous cycles
- age of the woman over 35
PICSI (presected sperm intracytoplasmic sperm injection)
It is a modified ICSI method in which only ripe sperm is used for fertilization of ovum. Ripe sperm has significantly lower rate of chromosomal abnormality, which decreases the risk of abortions.
Indications:
- spermogram rates on the bottom of standard values
- low fertilization after ICSI
- negative development of embryos in previous cycles
- repeated abortions in medical history of the couple
- age of the woman over 35
